DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
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Publication date
2017
Authors
Harterink, Martin
da Silva, Marta Esteves
Will, Lena
Turan, Julia
Ibrahim, Adiljan
Lang, Alexander E
van Battum, Eljo Y
Pasterkamp, R. Jeroen
Kapitein, Lukas C
Kudryashov, Dmitri
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Document Type
Article
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taverne
Abstract
The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
Keywords
ADP Ribose Transferases, Actin Cytoskeleton, Actins, Animals, Fibroblasts, Gelsolin, Green Fluorescent Proteins, HeLa Cells, Humans, Peptides, Rats, Recombinant Fusion Proteins, Transfection, Virulence Factors, Journal Article, Taverne, Biotechnology, Molecular Biology, Biochemistry, Cell Biology, Journal Article
Citation
Harterink, M, da Silva, M E, Will, L, Turan, J, Ibrahim, A, Lang, A E, van Battum, E Y, Pasterkamp, R J, Kapitein, L C, Kudryashov, D, Barres, B A, Hoogenraad, C C & Zuchero, J B 2017, 'DeActs : genetically encoded tools for perturbing the actin cytoskeleton in single cells', Nature Methods, vol. 14, no. 5, pp. 479-482. https://doi.org/10.1038/nmeth.4257