Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake
Publication date
1998
Authors
Zwart, G.
Huismans, R.
Agterveld, M.P. van
Peer, Y. van der
Rijk, P. de
Eenhoorn, H.
Muyzer, G.
Hannen, E.J. van
Gons, H.J.
Laanbroek, H.J.
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Document Type
Article
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Abstract
Bacterial diversity in the water column of a freshwater lake in the Netherlands was investigated by analysis of 16S rRNA
gene sequences recovered through PCR amplification from total community DNA. Among 23 unique cloned sequences, two
appeared to belong to the recently described bacterial division Verrucomicrobiales. One of the two sequences was most similar
to a group of environmental clones that form a distinct lineage within the division. The other sequence was divergent (less than
85% similarity) from all 16S rRNA gene sequences, both from cultivated species and from environmental clones, known in this
division to date. Analysis by denaturing gradient gel electrophoresis (DGGE) and sequencing of DNA recovered through
excision from the DGGE gel showed that the two sequence types were present in the lake throughout the year.
Keywords
Bacterial diversity, Freshwater pelagic zone, Denaturing gradient gel electrophoresis, Phylogeny, 16S rRNA, Verrucomicrobium, Prosthecobacter