Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection
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Publication date
2017-08-16
Authors
Gahtory, Digvijay
Murtola, Merita
Smulders, Maarten M J
Wennekes, Tom
Zuilhof, Han
Strömberg, Roger
Albada, H.B.
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Document Type
Article
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taverne
Abstract
In this report, we show how a convenient on-resin copper-click functionalization of azido-functionalized peptide nucleic acids (PNAs) allows various PNA-based detection strategies. Firstly, a thiazole orange (TO) clicked PNA probe facilitates a binary readout when combined with F/Q labeled DNA, giving increased sensitivity for antisense detection. Secondly, our TO-PNA conjugate also allows single nucleotide polymorphism detection. Since antisense detection is also possible in the absence of the TO label, our sensing platform based on azido-d-ornithine containing PNA even allows for additional and more advanced functionalization and sensing strategies.
Keywords
Azides, Benzothiazoles, Copper, DNA, Antisense, Molecular Probes, Peptide Nucleic Acids, Polymorphism, Single Nucleotide, Quinolines, Taverne
Citation
Gahtory, D, Murtola, M, Smulders, M M J, Wennekes, T, Zuilhof, H, Strömberg, R & Albada, B 2017, 'Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection', Organic and Biomolecular Chemistry, vol. 15, no. 32, pp. 6710-6714. https://doi.org/10.1039/c7ob01592e