Automated proton titrations of pancreatic phospholipase A2

Abstract

A computer-controlled titration system has been developed, tested, and used for analysis of the proton-titration behavior of pancreatic phospholipase A2 from ox, horse, and pig. The results revealed an error in the reported amino acid composition of the equine enzyme. A simple interface for the input of digitized data from a pH meter is described together with the software developed for controlling the titration experiments.