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CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

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1_s2.0_S1934590920305087_main.pdf (3.24 MB)

Publication date

2020-11-05

Authors

Hendriks, Delilah
Clevers, HansISNI 0000000043961208
Artegiani, Benedetta

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DOI

https://doi.org/10.1016/j.stem.2020.10.014

Document Type

Article

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taverne

Abstract

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

Keywords

Taverne, Genetics, Molecular Medicine, Cell Biology, Review, Journal Article

Citation

Hendriks, D, Clevers, H & Artegiani, B 2020, 'CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids', Cell stem cell, vol. 27, no. 5, pp. 705-731. https://doi.org/10.1016/j.stem.2020.10.014

URI

http://hdl.handle.net/1874/441505