Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping
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Publication date
2015-07-15
Authors
Jordan, Nan Yeun
Mimpen, Jolet Y.
van den Bogaard, Willie J M
Flesch, Frits M
van de Meent, M.H.M.
Sastre Torano, Javier
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Document Type
Article
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taverne
Abstract
Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6 min. The method was validated from 0.05 to 5 mu g mL(-1) CAF and 0.025-2.5 mu g mL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions
Keywords
Caffeine, Paraxanthine, CYP1A2, Phenotyping, Saliva, UHPLC, IN-VIVO, PLASMA, THEOPHYLLINE, METABOLITES, CYP2E1, RATIO, DRUG, Taverne
Citation
Jordan, N Y, Mimpen, J Y, van den Bogaard, W J M, Flesch, F M, van de Meent, M H M & Torano, J S 2015, 'Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping', Journal of chromatography. B, vol. 995, pp. 70-73. https://doi.org/10.1016/j.jchromb.2015.05.020