Separation of sialyl-oligosaccharides by medium pressure anion-exchange chromatography on Mono Q®

Abstract

On columns prepacked with the recently introduced anion-exchange material, Mono Q® (Pharmacia), sialyl-oligosaccharides could be fractionated excellently according to the sialic acid content. With u.v. absorption at 214 nm as the detection method, analytical runs of carbohydrate material on the microgram scale, were possible. The value of the method for preparative purposes was demonstrated for sialic acid-containing carbohydrates obtained from human serotransferrin by hydrazinolysis.