Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing
Publication date
2022-12-16
Authors
van Asperen, Jessy V.
van Bodegraven, Emma J
Robe, Pierre A J T
Hol, Elly
Editors
Advisors
Supervisors
Document Type
Article
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License
cc_by
Abstract
The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).
Keywords
Antibody, Cancer, Cell biology, Microscopy, Neuroscience, General Biochemistry,Genetics and Molecular Biology, General Immunology and Microbiology, General Neuroscience, Journal Article
Citation
van Asperen, J V, van Bodegraven, E J, Robe, P A J T & Hol, E M 2022, 'Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing', STAR protocols, vol. 3, no. 4, 101703. https://doi.org/10.1016/j.xpro.2022.101703