Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes
Publication date
2021-03-19
Authors
Maas, Renee G C
Lee, Soah
Harakalova, Magdalena
Snijders Blok, Christian J.B.
Goodyer, William R.
Hjortnaes, Jesper
Doevendans, Pieter
van Laake, Linda W
van der Velden, Joanne M.
Asselbergs, Folkert W
Editors
Advisors
Supervisors
Document Type
Article
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License
cc_by_nc_nd
Abstract
Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule inhibition with CHIR99021. The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream assays such as disease modeling, drug and toxicity screening, and cell therapy. For complete details on the use and execution of this protocol, please refer to Buikema (2020).
Keywords
Cell culture, Cell differentiation, Stem cells, General Biochemistry,Genetics and Molecular Biology, General Neuroscience, General Immunology and Microbiology
Citation
Maas, R G C, Lee, S, Harakalova, M, Snijders Blok, C J B, Goodyer, W R, Hjortnaes, J, Doevendans, P A F M, Van Laake, L W, van der Velden, J, Asselbergs, F W, Wu, J C, Sluijter, J P G, Wu, S M & Buikema, J W 2021, 'Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes', STAR protocols, vol. 2, no. 1, 100334. https://doi.org/10.1016/j.xpro.2021.100334