Water soluble lipid precursor contaminants in yeast culture medium ingredients

Publication date

2020-06-27

Authors

Renne, Mike F.ISNI 0000000443826953
Bao, X.ORCID 0000-0002-5129-0728ISNI 000000049281246X
de Kroon, A.I.P.M.ISNI 0000000390026724

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Abstract

The presence of the water soluble glycerophospholipid precursors choline and inositol in culture media highly affects lipid biosynthesis and regulation thereof. We report that widely used media ingredients contain trace amounts of choline and inositol that are not mentioned on the product label, influencing experimental outcome.

Keywords

Choline, Culture medium ingredients, Inositol, Lipid metabolism, Saccharomyces cerevisiae, Soluble lipid precursors

Citation

Renne, M F, Bao, X & de Kroon, A I P M 2020, 'Water soluble lipid precursor contaminants in yeast culture medium ingredients', FEMS Yeast Research, vol. 20, no. 5, foaa029. https://doi.org/10.1093/femsyr/foaa029