Electrophoretic comparison of nuclear and nucleolar proteins : I. Beef Pancreas

Abstract

Nuclei and nucleoli, isolated from beef pancreas, are extracted with 0.14 M NaCl, 1 M NaCl and 0.1 N NaOH in the order given. The 0.14 M NaCl and 0.1 N NaOH extracts lend themselves to paper and agar electrophoresis and the electropherograms obtained from these extracts of nuclei and nucleoli are compared. It is shown that the proteins of the nucleoli are in several aspects different from the proteins of the non-nucleolar part of the nucleus.