Metabolic Engineering toward Sustainable Production of Nylon-6
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Publication date
2016-01-15
Authors
Turk, Stefan C H J
Kloosterman, Wigard P.
Ninaber, Dennis K.
Kolen, Karin P A M
Knutova, Julia
Suir, Erwin
Schürmann, Martin
Raemakers-Franken, Petronella C.
Müller, Monika
De Wildeman, Stefaan M A
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taverne
Abstract
Nylon-6 is a bulk polymer used for many applications. It consists of the non-natural building block 6-aminocaproic acid, the linear form of caprolactam. Via a retro-synthetic approach, two synthetic pathways were identified for the fermentative production of 6-aminocaproic acid. Both pathways require yet unreported novel biocatalytic steps. We demonstrated proof of these bioconversions by in vitro enzyme assays with a set of selected candidate proteins expressed in Escherichia coli. One of the biosynthetic pathways starts with 2-oxoglutarate and contains bioconversions of the ketoacid elongation pathway known from methanogenic archaea. This pathway was selected for implementation in E. coli and yielded 6-aminocaproic acid at levels up to 160 mg/L in lab-scale batch fermentations. The total amount of 6-aminocaproic acid and related intermediates generated by this pathway exceeded 2 g/L in lab-scale fed-batch fermentations, indicating its potential for further optimization toward large-scale sustainable production of nylon-6.
Keywords
6-aminocaproic acid, adipate, caprolactam, metabolic engineering, nylon-6, α-ketopimelate, Taverne, Biochemistry, Genetics and Molecular Biology (miscellaneous), Biomedical Engineering, Journal Article
Citation
Turk, S C H J, Kloosterman, W P, Ninaber, D K, Kolen, K P A M, Knutova, J, Suir, E, Schürmann, M, Raemakers-Franken, P C, Müller, M, De Wildeman, S M A, Raamsdonk, L M, Van Der Pol, R, Wu, L, Temudo, M F, Van Der Hoeven, R A M, Akeroyd, M, Van Der Stoel, R E, Noorman, H J, Bovenberg, R A L & Trefzer, A C 2016, 'Metabolic Engineering toward Sustainable Production of Nylon-6', ACS Synthetic Biology, vol. 5, no. 1, pp. 65-73. https://doi.org/10.1021/acssynbio.5b00129