Imaging translation dynamics of single mRNA molecules in live cells
Publication date
2018
Authors
Ruijtenberg, Suzan
Hoek, Tim A.
Yan, Xiaowei
Tanenbaum, Marvin E.
Editors
Advisors
Supervisors
Document Type
Part of book
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taverne
Abstract
mRNA translation is a key step in decoding the genetic information stored in DNA. Regulation of translation efficiency contributes to gene expression control and is therefore important for cell fate and function. Here, we describe a recently developed microscopy-based method that allows for visualization of translation of single mRNAs in live cells. The ability to measure translation dynamics of single mRNAs will enable a better understanding of spatiotemporal control of translation, and will provide unique insights into translational heterogeneity of different mRNA molecules in single cells.
Keywords
Fluorescence, Microscopy, mRNA, Single molecule imaging, SunTag, Translation, Taverne, Molecular Biology, Genetics
Citation
Ruijtenberg, S, Hoek, T A, Yan, X & Tanenbaum, M E 2018, Imaging translation dynamics of single mRNA molecules in live cells. in Methods in Molecular Biology. vol. 1649, Methods in Molecular Biology, vol. 1649, Humana Press Inc., pp. 385-404. https://doi.org/10.1007/978-1-4939-7213-5_26