Log In

Protein 4.1G binds to a unique motif within the Fc gamma RI cytoplasmic tail

Files

JL Protein 4.pdf (986.69 KB)

Publication date

2008-04

Authors

Beekman, JeffreyISNI 0000000388915338
Bakema, J.E.
van der Poel, C.E.
van der Linden, J.A.
Van De Winkel, Jan G.J.ISNI 0000000083658940
Leusen, JeanetteORCID 0000-0003-4982-6914ISNI 0000000390807686

Editors

Advisors

Supervisors

DOI

https://doi.org/10.1016/j.molimm.2007.10.024

Document Type

Article

Metadata

Show full item record

Collections

Utrecht University Repository
UMC Repository
Open Access logo

License

Abstract

The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternatively spliced 4.1G product to be associated with increased Fc gamma RI binding in yeast two-hybrid assays, and to be selectively enriched in most immune cells at the transcript level. In addition, a detailed analysis of the 4.1G 'docking site' within Fc gamma RI is provided by examining Fc gamma RI-CY-truncated and alanine-substituted mutants. These pointed to an Fc gamma RI membrane-proximal core motif of HxxBxxxBB (H represents hydrophobic residues, B basic residues and x represents any residue), followed by hydrophobic and (potentially) negatively charged residues to be central for interaction with protein 4.1G.

Keywords

Alternative Splicing, Amino Acid Motifs, Amino Acid Sequence, Animals, Cell Line, Cell Membrane, Cytoplasm, Cytoskeletal Proteins, Humans, Immunoprecipitation, Membrane Proteins, Mice, Molecular Sequence Data, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, RNA, Messenger, Receptors, IgG, Recombinant Proteins, Sequence Homology, Amino Acid, Two-Hybrid System Techniques, Journal Article, Research Support, Non-U.S. Gov't, Journal Article, Research Support, Non-U.S. Gov't

Citation

Beekman, J M, Bakema, J E, van der Poel, C E, van der Linden, J A, van de Winkel, J G J & Leusen, J H W 2008, 'Protein 4.1G binds to a unique motif within the Fc gamma RI cytoplasmic tail', Molecular Immunology, vol. 45, no. 7, pp. 2069-2075. https://doi.org/10.1016/j.molimm.2007.10.024

URI

https://dspace.library.uu.nl/handle/1874/32109