Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium
Publication date
2019-12-01
Authors
Top, Janetta
Hendrickx, Antoni P. A.
van Ampting, Marleen T.J.
van Limpt, Kees
Knol, Jan
van de Kamer, Denise
Braat, Johanna C.
Viveen, Marco C
Rogers, Malbert
Kemperman, Hans
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Advisors
Supervisors
Document Type
Article
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Abstract
The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.
Keywords
Enterococcus faecium, gut colonization, low-calcium diet, mouse model, Microbiology
Citation
Top, J, Hendrickx, A P A, van Ampting, M T J, van Limpt, K, Knol, J, van de Kamer, D, Braat, J C, Viveen, M, Rogers, M R, Kemperman, H, Willems, R J L & Paganelli, F L 2019, 'Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium', MicrobiologyOpen, vol. 8, e936. https://doi.org/10.1002/mbo3.936