In vitro reconstitution of dynamic microtubules interacting with actin filament networks

Abstract

Interactions between microtubules and actin filaments (F-actin) are essential for eukaryotic cell migration, polarization, growth, and division. Although the importance of these interactions has been long recognized, the inherent complexity of the cell interior hampers a detailed mechanistic study of how these two cytoskeletal systems influence each other. In this chapter, we show how in vitro reconstitution can be employed to study how actin filaments and dynamic microtubules affect each other's organization. While we focus here on the effect of steric interactions, these assays provide an ideal starting point to develop more complex studies through the addition of known F-actin–microtubule cross-linkers, or myosin II motors.