Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus.
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Publication date
2014
Authors
Raj, V Stalin
Smits, Saskia L
Provacia, Lisette B
van den Brand, Judith
Wiersma, Lidewij
Ouwendijk, Werner J D
Bestebroer, Theo M
Spronken, Monique I
van Amerongen, Geert
Rottier, P.J.M.
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Article
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Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection.
Keywords
Molecular Sequence Data, Protein Binding, Receptors, Virus, Adenosine Deaminase: metabolism, Amino Acid Sequence, Animals, Coronaviridae, Coronaviridae Infections, Coronaviridae Infections: enzymology, Coronaviridae Infections: virology, Coronaviridae: genetics, Coronaviridae: physiology, Dipeptidyl Peptidase 4, Dipeptidyl Peptidase 4: chemistry, Adenosine Deaminase, Virus Internalization, Spike Glycoprotein, Coronavirus: metabolism, Spike Glycoprotein, Coronavirus: genetics, Spike Glycoprotein, Coronavirus, Sequence Alignment, Receptors, Virus: metabolism, Adenosine Deaminase: genetics, Receptors, Virus: genetics, Receptors, Virus: chemistry, Dipeptidyl Peptidase 4: genetics, Dipeptidyl Peptidase 4: metabolism, Disease Models, Animal, Ferrets, Humans
Citation
Raj, V S, Smits, S L, Provacia, L B, van den Brand, J M A, Wiersma, L, Ouwendijk, W J D, Bestebroer, T M, Spronken, M I, van Amerongen, G, Rottier, P J M, Fouchier, R A M, Bosch, B J, Osterhaus, A D M E & Haagmans, B L 2014, 'Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus.', Journal of Virology, vol. 88, no. 3, pp. 1834-8. https://doi.org/10.1128/JVI.02935-13