Thin layer isoelectric focusing of the soluble lens extracts from larval stages and adult Xenopus laevis

Abstract

Soluble lens extracts from different larval stages and also from adult Xenopus laevis were studied by thin layer isoelectric focusing. These lens extracts were also tested against antiserum to adult X. laevis total lens proteins by two-dimensional antigen-antibody crossed electrophoresis. By isoelectric focusing the lens crystallins were resolved into 13 major components. In the adult lens some components of the γ-crystallins were lost, the β-crystallins formed the major fraction and the α-crystallin appeared to be the weakest of the crystallin fractions.