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Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR

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Publication date

2013

Authors

Pacholewicz, E.
Swart, A.
Lipman, L.J.A.
Wagenaar, J.A.
Havelaar, A.H.
Duim, B.

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DOI

https://doi.org/10.1016/j.mimet.2013.06.003

Document Type

Article

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Utrecht University Repository

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(c) UU Universiteit Utrecht, 2013

Abstract

A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.

Keywords

Campylobacter, qPCR, PMA, Broiler

Citation

URI

http://hdl.handle.net/1874/287268