Disease-Suppressive Soils Induce Systemic Resistance in Arabidopsis thaliana Against Pseudomonas syringae pv. tomato

Abstract

Arabidopsis thaliana accession Col-0 seedlings were transferred into an autoclaved soil/sand mixture amended with either 10 or 20% (wt/wt) soil from fields in Washington State that are suppressive to take-all or Rhizoctonia root rot of wheat. Three weeks after transplanting, these plants had population sizes of 2,4-diacetylphloroglucinol (DAPG) or phenazine-1-carboxylic acid (PCA)-producing pseudomonads of greater than 5 × 105 colony forming units per gram fresh weight of root with rhizosphere soil. When the plants were challenge-inoculated with Pseudomonas syringae pv. tomato DC3000, both soils induced systemic resistance in the leaves against bacterial speck to a level similar to that induced by P. simiae WCS417r, P. fluorescens Q2-87 (DAPG+), P. brassicacearum Q8r1-96 and L5.1-96 (both DAPG+), and P. synxantha 2-79 (PCA+). Pasteurization of the soils before adding them into the soil/sand mixture eliminated DAPG- and PCA-producing pseudomonads from the Arabidopsis rhizosphere and significantly reduced induced systemic resistance activity. However, populations of total culturable aerobic bacteria were similar in the rhizosphere of plants grown in soil/sand mixes amended with untreated or pasteurized suppressive soils. This is the first report of induction of systemic resistance in Arabidopsis by take-all and Rhizoctonia-suppressive soils and the ability of PCA-producing P. synxantha 2-79 to induce resistance.