Molecular species of extracellular phosphatidylethanolamine from Escherichia coli

Abstract

1. (1)|Phosphatidylethanolamine was hydrolysed by phospholipase C from Bacillus cereus (E.C. 3.1.4.3) and the resultant diglycerides were separated into five subfractions on thin-layer plates of silica impregnated with silver nitrate.

2. (2)|The positional distribution of the fatty acids in these diglycerides was determined by means of hydrolysis with pancreatic lipase (E.C. 3.1.1.3). The results were in good agreement with those obtained by hydrolysis of the phosphatidylethanolamine with snake-venom phospholipase A (E.C. 3.1.1.4).

3. (3)|The following molecular species accounting for 97.3% of the phosphatidylethanolamine could be calculated: (1-octadecenoyl-2-hexadecenoyl)-, (di-octadecenoyl)-, (1-palmitoyl-2-hexadecenoyl)-, (1-hexadecenoyl-2-palmitoyl), (1-palmitoyl-2-octadecenoyl)-, (1-octadecenoyl-2-palmitoyl)-, (1-palmitoyl-2-cis-3, 10-methylene-hexadecanoyl)-, and (di-palmitoyl)-phosphatidylethanolamine.