Structure determination of the major asparagine-linked sugar chain of human factor VIII - von Willebrand factor
Publication date
1983
Authors
Vliegenthart, J.F.G.
Debeire, P.
Montreuil, J.
Samor, B.
Mazurier, C.
Goudemand, M.
Halbeek, H. van
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DOI
Document Type
Article
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Abstract
N-glycosidically-linked glycans released by hydrazinolysis of human factor VIII/von Willebrand factor (FVIII/vWf) were seperated by high-voltage electrophoresis. Five fraction were obtained, one of them representing 60% of the total amount of the N-glycosidically-linked glycans of FVIII/vWf. On the basis of the carbohydrate composition, methylation analysis and 500 MHz 1H-NMR spectroscopy, we describe the primary structure of this major glycan which is of the monosialylated and monofucosylated biantennary N-acetyllactosaminic type.