Intercropping with Trifolium repens contributes disease suppression of banana Fusarium wilt by reshaping soil protistan communities

Abstract

Fusarium wilt disease of bananas, caused by the soil-borne pathogen Fusarium oxysporum, threatens banana production. Intercropping, cultivation of more than one crop simultaneously on the same field, has emerged as efficient and sustainable land management for suppressing Fusarium wilt disease. Although previous studies have proven the changes in soil microbial communities including bacteria and fungi under intercropping contributed to disease suppression, little is known about the role of protistan communities in driving this effect. In a field experiment, we assessed microbiome shifts with a focus on protists under intercropping of banana with the legume Trifolium repens. Our results showed that the reduced Fusarium wilt disease incidence and the decreased Fusarium pathogen density under intercropping could be attributed to the changes in protistan community compositions. Specially, we observed a significant negative correlation between the relative abundance of phagotrophic protists and Fusarium oxysporum. We further conducted a pot experiment to examine the impacts of the legume crop on phagotrophic protists across different spatial distances, which showing that intercropping enriched the relative abundance of phagotrophic protists, especially Cercomonas, with members of this group showing the capacity to directly inhibit the growth of Fusarium pathogen in vitro. We highlight that predatory protists are important agents underlying disease suppression in intercropping system, which can offer new venues to promote plant health in sustainable agriculture.