Genetic characterization of the DNA of the bacteriophage φX174 70 S particle

Publication date

1972-05

Authors

Weisbeek, P.J.
Pol, J.H. van de
Arkel, G.A. van

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Abstract

The DNA of the 70 S particle of the bacteriophage φX174 has been characterized. By hybridization of RF II DNA of mutants from five different cistrons with DNA from wild-type 70 S particles it is demonstrated that this latter DNA is heterogeneous. Although the molecular weight is about 20% of the intact φX DNA, the 70 S DNA preparation contains nucleotide sequences from all five cistrons tested. The 70 S DNA turns out to consist of the plus strand type.

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