Characterization of D-xylose reductase, XyrB, from Aspergillus niger
Publication date
2021-06
Authors
Terebieniec, Agata
Chroumpi, Tania
Dilokpimol, Adiphol
Aguilar-Pontes, Maria Victoria
Mäkelä, Miia R.
de Vries, Ronald
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Document Type
Article
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Abstract
D-xylose reductase is a member of the aldo-keto reductase family, and is involved in D-xylose and L-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second D-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert D-xylose and L-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).
Keywords
Aspergillus niger, D-xylose reductase, Pentose Catabolic Pathway, Biotechnology, Applied Microbiology and Biotechnology
Citation
Terebieniec, A, Chroumpi, T, Dilokpimol, A, Aguilar-Pontes, M V, Mäkelä, M R & de Vries, R P 2021, 'Characterization of D-xylose reductase, XyrB, from Aspergillus niger', Biotechnology Reports, vol. 30, e00610, pp. 1-8. https://doi.org/10.1016/j.btre.2021.e00610