Fitness and virulence of a coxsackievirus mutant that can circumnavigate the need for phosphatidylinositol 4-kinase class III beta
Publication date
2014
Authors
Thibaut, Hendrik Jan
van der Schaar, Hilde M
Lanke, Kjerstin H W
Verbeken, Erik
Andrews, Martin
Leyssen, Pieter
Neyts, Johan
van Kuppeveld, Frank J M
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Supervisors
Document Type
Article
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Abstract
Coxsackieviruses require phosphatidylinositol-4-kinase IIIβ (PI4KIIIβ) for replication but can bypass this need by an H57Y mutation in protein 3A (3A-H57Y). We show that mutant coxsackievirus is not outcompeted by wild-type virus during 10 passages in vitro. In mice, the mutant virus proved as virulent as wild-type virus, even when mice were treated with a PI4KIIIβ inhibitor. Our data suggest that upon emergence, the 3A-H57Y mutant has the fitness to establish a resistant population with a virulence similar to that of wild-type virus.
Keywords
1-Phosphatidylinositol 4-Kinase, Animals, Coxsackievirus Infections, Enterovirus, Genetic Fitness, Host-Pathogen Interactions, Mice, Mutation, Viral Proteins, Virulence, Virus Replication, Coronacrisis-Taverne
Citation
Thibaut, H J, van der Schaar, H M, Lanke, K H W, Verbeken, E, Andrews, M, Leyssen, P, Neyts, J & van Kuppeveld, F J M 2014, 'Fitness and virulence of a coxsackievirus mutant that can circumnavigate the need for phosphatidylinositol 4-kinase class III beta', Journal of Virology, vol. 88, no. 5, pp. 3048-3051. https://doi.org/10.1128/JVI.03177-13