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Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

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rabouille.pdf (350.44 KB)

Publication date

2018

Authors

Rabouille, CatherineISNI 0000000001502935

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DOI

https://doi.org/10.1007/978-1-4939-7213-5_11

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Part of book

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Abstract

The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.

Keywords

Electron microscopy, Frozen sections, ISH-IEM, Immunoelectron microscopy, In situ hybridization, Ultrastructure, mRNA detection, Taverne, Journal Article

Citation

Rabouille, C 2018, Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections. in RNA Detection : methods and protocols. Methods in molecular biology, vol. 1649, Humana Press, pp. 177-186. https://doi.org/10.1007/978-1-4939-7213-5_11

URI

http://hdl.handle.net/1874/363442