Differential uptake of nanoparticles by human M1 and M2 polarized macrophages: Protein corona as a critical determinant
Publication date
2016-11-01
Authors
Binnemars-Postma, Karin A.
Ten Hoopen, Hetty Wm
Storm, Gert
Prakash, Jai
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Supervisors
Document Type
Article
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taverne
Abstract
Aim: To investigate the interaction behavior of M1- and M2-type macrophages with nanoparticles of different sizes with/without the presence of serum. Materials & methods: THP-1 human monocytes were differentiated into M1 and M2 macrophages, and the uptake of silica nanoparticle (50-1000 nm) was studied using flow cytometry and different microscopies. Results: Without serum, higher uptake of all-sized nanoparticles was observed by M1 compared with M2. With serum, uptake of nanoparticles (200-1000 nm) was dramatically increased by M2. Furthermore, serum proteins adsorbed (corona) by nanoparticles were found to be the ligands for receptors expressed by M2, as revealed by SDS-PAGE and gene profiling analyses. Conclusion: The observed differential uptake by M1 and M2 macrophages will help understand the fate of nanoparticles in vivo.
Keywords
complement factor, heat-inactivated serum, M1 macrophages, M2 macrophages, nanoparticles, opsonization, phagocytosis, protein corona, serum proteins, silica nanoparticles, Taverne, Medicine (miscellaneous), Bioengineering, Biomedical Engineering, Development, General Materials Science
Citation
Binnemars-Postma, K A, Ten Hoopen, H W, Storm, G & Prakash, J 2016, 'Differential uptake of nanoparticles by human M1 and M2 polarized macrophages : Protein corona as a critical determinant', Nanomedicine, vol. 11, no. 22, pp. 2889-2902. https://doi.org/10.2217/nnm-2016-0233