Structural analysis of acidic oligosaccharides derived from the methylated, acidic polysaccharide associated with coccoliths of Emiliania huxleyi (lohmann) kamptner

Abstract

A series of acidic oligosaccharides was obtained by graded, acid hydrolysis of the methylated, acidic polysaccharide associated with the coccoliths of the alga Emiliania huxleyi (Lohmann) Kamptner. After fractionation by ion-exchange chromatography, the structures of the oligosaccharides were determined by sugar analysis, g.l.c.-m.s. of the intact, permethylated oligosaccharide-alditols, and methylation analysis. The following oligosaccharides were characterised:

α--GalpA-(1→6)-α--Manp-(1→3)--Man, -GalpA-(1→4)--GalpA-(1→6)-Man, -GalpA-(1→4)--GalpA-(1→2/6)-Manp-(1→3)-Man, -GalpA-(1→4)--GalpA-(1→3)--Xyl, -GalpA-(1→2)--Manp6Me-(1→4)--GalpA-(1→2)--Rha, -GalpA-(1→2)-Manp-(1→4)--GalpA-(1→2)--Rha, -GalpA-(1→2)--Rhap-(1→4)--GalpA-(1→2)--Rha, -GalpA-(1→2)--Manp6Me-(1→4)--GalA, -GalpA-(1→2)-Manp-(1→4)--GalA, -GalpA-(1→2)--Manp6Me-(1→4)--GalpA-(1→2)--Man6Me, -GalpA-(1→2)--Manp6Me-(1→4)--GalpA-(1→2)-Man, -GalpA3Me-(1→2)--Manp6Me-(1→4)--GalpA-(1→2)--Man6Me, and -GalpA3Me-(1→2)--Manp6Me-(1→4)--GalA.