Effect of growth-promoting 17beta-estradiol, 19-nortestosterone and dexamethasone on circulating levels of nine potential biomarker candidates in veal calves.
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Publication date
2009
Authors
Cacciatore, G.
Eisenberg, S W F
Situ, C.
Mooney, M.H.
Delahaut, P.
Klarenbeek, S.
Huet, A.C.
Bergwerff, Aldert A
Elliott, C.T.
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Abstract
The use of screening methods based on the detection of biological effects of growth promoters is a promising approach to assist residue monitoring. To reveal useful effects on protein metabolism, male and female veal calves at 10 weeks of age were treated thrice with a combination of 25mg 17beta-estradiol 3-benzoate and 150 mg 19-nortestosterone decanoate with 2 weeks intervals and finally once with 4 mg dexamethasone. Hormone-treated calves showed a significant accelerated growth rate over 6 weeks. Plasma samples of treated and control calves were analysed for immunoreactive inhibin (ir-inhibin), osteocalcin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor-binding protein 2 (IGFBP-2), IGFBP-3, luteinzing hormone (LH), follicle-stimulating hormone (FSH) and prolactin using immunoaffinity assays. Hormone treatment did not affect levels of IGF-1, IGFBP-2, IGFBP-3, LH, FSH and prolactin. The concentration of circulating ir-inhibin decreased, however, significantly (P
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Cacciatore, G, Eisenberg, S W, Situ, C, Mooney, M H, Delahaut, P, Klarenbeek, S, Huet, A C, Bergwerff, A A & Elliott, C T 2009, 'Effect of growth-promoting 17beta-estradiol, 19-nortestosterone and dexamethasone on circulating levels of nine potential biomarker candidates in veal calves.', Analytica Chimica Acta, vol. 637, no. 1-2, pp. 351-359.