Inverse polymerase chain reaction for rapid gene isolation in Arabidopsis thaliana insertion mutants

Abstract

Recently, many mutants have been isolated in the model plant Arabidopsis thaliana by the insertion of the Agrobacterium tumefaciens T-DNA into the plant genome. Instead of applying Southern analysis on these insertion mutants and to avoid the construction of mutant- derived genomic libraries, we propose a much shorter and less laborious strategy for cloning the wild-type genes corresponding to the mutant loci by making use of the polymerase chain reaction (PCR) technology.