WDR47 protects neuronal microtubule minus ends from katanin-mediated severing
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Publication date
2021-07-13
Authors
Buijs, Robin R.
Hummel, Jessica Ja
Burute, Mithila
Pan, Xingxiu
Cao, Yujie
Stucchi, Riccardo
Altelaar, A.F. Maarten
Akhmanova, Anna
Kapitein, Lukas
Hoogenraad, Casper
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Article
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Abstract
Axons and dendrites are long extensions of neurons that contain arrays of noncentrosomal microtubules. Calmodulin-regulated spectrin-associated proteins (CAMSAPs) bind to and stabilize free microtubule minus ends and are critical for proper neuronal development and function. Previous studies have shown that the microtubule-severing ATPase katanin interacts with CAMSAPs and limits the length of CAMSAP-decorated microtubule stretches. However, how CAMSAP and microtubule minus end dynamics are regulated in neurons is poorly understood. Here, we show that the neuron-enriched protein WDR47 interacts with CAMSAPs and is critical for axon and dendrite development. We find that WDR47 accumulates at CAMSAP2-decorated microtubules, is essential for maintaining CAMSAP2 stretches, and protects minus ends from katanin-mediated severing. We propose a model where WDR47 protects CAMSAP2 at microtubule minus ends from katanin activity to ensure proper stabilization of the neuronal microtubule network.
Keywords
axon, CAMSAP, centrosome, cytoskeleton, dendrite, katanin, microtubule, neuron, WDR47, General Biochemistry,Genetics and Molecular Biology
Citation
Buijs, R R, Hummel, J J A, Burute, M, Pan, X, Cao, Y, Stucchi, R, Altelaar, M, Akhmanova, A, Kapitein, L C & Hoogenraad, C C 2021, 'WDR47 protects neuronal microtubule minus ends from katanin-mediated severing', Cell Reports, vol. 36, no. 2, 109371, pp. 1-e7. https://doi.org/10.1016/j.celrep.2021.109371