Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid

Abstract

The commonly used 3% sulphosalicylic acid method for the determination of protein in spinal fluid does not give satisfactory results, and two other methods are described. The first consists of the addition of 7% sodium sulphate to the 3% sulphosalicylic acid. The second method consists of the use of a 3% trichloroacetic acid solution in water only. Both give good results comparable with the biuret and the Kafka methods for total protein in spinal fluids.