A bioassay using intestinal organoids to measure CFTR modulators in human plasma
Publication date
2015
Authors
Dekkers, R
Vijftigschild, L A W
Vonk, A M
Kruisselbrink, E
de Winter-de Groot, K M
Janssens, H M
van der Ent, C K
Beekman, J M
van der Ent, Kors
Editors
Advisors
Supervisors
Document Type
Article
Metadata
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License
taverne
Abstract
Treatment efficacies of drugs depend on patient-specific pharmacokinetic and pharmacodynamic properties. Here, we developed an assay to measure functional levels of the CFTR potentiator VX-770 in human plasma and observed that VX-770 in plasma from different donors induced variable CFTR function in intestinal organoids. This assay can help to understand variability in treatment response to CFTR potentiators by functionally modeling individual pharmacokinetics.
Keywords
Taverne, Journal Article, Research Support, Non-U.S. Gov't
Citation
Dekkers, R, Vijftigschild, L A W, Vonk, A M, Kruisselbrink, E, de Winter-de Groot, K M, Janssens, H M, van der Ent, C K, Beekman, J M & van der Ent, CK 2015, 'A bioassay using intestinal organoids to measure CFTR modulators in human plasma', Journal of Cystic Fibrosis, vol. 14, no. 2, pp. 178-181. https://doi.org/10.1016/j.jcf.2014.10.007