A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization
Publication date
2017
Authors
van Beurden, S.J.
IJsseldijk, L.L.
van de Bildt, Marco W G
Begeman, L
Wellehan, James F X
Waltzek, Thomas B
de Vrieze, Geert
Gröne, A.
Kuiken, Thijs
Verheije, Hélène
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.
Keywords
Intestinal Conten, Bottlenose Dolphin, Harbour Porpoise, Protease Cleavage Site, Bowhead Whale, Taverne
Citation
van Beurden, S J, IJsseldijk, L L, van de Bildt, M W G, Begeman, L, Wellehan, J F X, Waltzek, T B, de Vrieze, G, Gröne, A, Kuiken, T, Verheije, M H & Penzes, J J 2017, 'A novel cetacean adenovirus in stranded harbour porpoises from the North Sea : detection and molecular characterization', Archives of Virology, vol. 162, no. 7, pp. 2035–2040. https://doi.org/10.1007/s00705-017-3310-8