An immunobioluminescence assay for gamma-gamma enolase activity in human serum and cerebrospinal fluid

Abstract

A solid phase immunobioluminescence assay for the measurement of gammagamma enolase activity in human serum and cerebrospinal fluid is described. The assay combines the specificity of the antiserum with the specificity of measuring enolase activity, thus making the assay highly specific for gamma-gamma enolase. The sensitivity of the method allows determinations in unconcentrated cerebrospinal fluid samples. The new immunobioluminescence assay has been compared with a radioimmunoassay method. The correlation is good both for serum and CSF samples. However, different correlation lines are found for serum and CSF.