Primary structure of a novel N-glycosidic carbohydrate unit, derived from hen ovomucoid
Files
Publication date
1983
Authors
Vliegenthart, J.F.G.
Paz Parente, J.
Strecker, G.
Leroy, Y.
Montreuil, J.
Fournet, B.
Halbeek, H. van
Dorland, L.
Editors
Advisors
Supervisors
DOI
Document Type
Article
Metadata
Show full item recordCollections
License
Abstract
The N-glycosidic carbohydrate chains of hen -ovomucoid were released from the protein by hydrazinolysis, and separated by HPLC. Primary structural analysis of 3 major fractions was conducted by applying 500-MHz 1H-NMR spectroscopy in combination with methylation analysis. One of the fractions investigated appeared to consist of an intersected penta-antennary structure extended with one Gal residue. The location of the latter in a certain branch could be established unambiguously by NMR.
This structure is a novel member of the family of N-glycosidic carbohydrates of glycoproteins.