Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry
Publication date
2025-12-19
Authors
Cardenas, Matias
Compton, Sasha
Hunt, Ashkon
Chopra, Pradeep
Perez, Daniel R
Boons, Geert-Jan
Rajao, Daniela S
Editors
Advisors
Supervisors
Document Type
Article
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License
cc_by_nc_nd
Abstract
Recent work has shown that major histocompatibility complex (MHC) class II is a functional receptor for some influenza A virus (FLUAV) subtypes. Here, we present a protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry. We describe steps for producing recombinant human leukocyte antigen DR isotype (HLA-DR) MHC class II using a baculovirus expression system. The HLA-DR antigen is then modified for its utilization as a biolayer interferometry (BLI) probe for kinetic experiments using whole FLUAV viruses. This protocol allows for the screening of different FLUAV subtypes and their affinity for MHC class II.
Keywords
General Neuroscience, General Immunology and Microbiology, General Biochemistry,Genetics and Molecular Biology
Citation
Cardenas, M, Compton, S, Hunt, A, Chopra, P, Perez, D R, Boons, G-J & Rajao, D S 2025, 'Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry', STAR Protocols, vol. 6, no. 4, 104155. https://doi.org/10.1016/j.xpro.2025.104155