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CRISPR/Cas 9 genome editing and its applications in organoids

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Publication date

2017-03-01

Authors

Driehuis, Else
Clevers, HansISNI 0000000043961208

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DOI

https://doi.org/10.1152/ajpgi.00410.2016

Document Type

Article

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taverne

Abstract

Organoids are three-dimensional (3D) structures derived from adult or embryonic stem cells that maintain many structural and functional features of their respective organ. Recently, genome editing based on the bacterial defense mechanism CRISPR/Cas9 has emerged as an easily applicable and reliable laboratory tool. Combining organoids and CRISPR/Cas9 creates exciting new opportunities to study organ development and human disease in vitro. The potential applications of CRISPR in organoids are only beginning to be explored.

Keywords

CRISPR/Cas9, gene editing, organoid, Taverne, Physiology, Hepatology, Gastroenterology, Physiology (medical), Journal Article, Review

Citation

Driehuis, E & Clevers, H 2017, 'CRISPR/Cas 9 genome editing and its applications in organoids', American Journal of Physiology-Gastrointestinal and Liver Physiology, vol. 312, no. 3, pp. G257-G265. https://doi.org/10.1152/ajpgi.00410.2016

URI

http://hdl.handle.net/1874/355528