1H-NMR and photochemically-induced dynamic nuclear polarization studies on bovine pancreatic phospholipase A2
Publication date
1980-06-26
Authors
Egmond, M.R.
Slotboom, A.J.
Haas, G.H. de
Dijkstra, Klaas
Kaptein, R.
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Document Type
Article
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Abstract
Proton-NMR resonances of trytophan 3 and tyrosine 69 in bovine pancreatic phospholipase A2, its pro-enzyme and in Ala1-transaminated protein were assigned using photochemically-induced dynamic nuclear polarization (photo-CIDNP) as such or in combination with spin-echo measurements. In addition assignments were made by suppression of cross-relaxation effects using short (0.1 s) high-power laser pulses.
Keywords
phospholipase A2, 1H-NMR, dynamic nuclear polarization, bovine pancreas