Nonlinear spectral imaging of fungi

Abstract

Nonlinear microscopy combined with fluorescence spectroscopy is known as nonlinear spectral imaging microscopy (NLSM). This method provides simultaneously specimen morphology – distinguishing different parts in a tissue – and (auto)fluorescence spectra, thus their biochemical composition. A novel data analysis method, the spectral phasor, allows extracting and identifying chemical components from these spectra. The phasor is a very fast and visually intuitive method requiring little input and exhibiting little bias.