Comparison of the specific conductivities, buffer, capacities and molecular weights of focused ampholine, servalyte and pharmalyte carrier ampholytes used in isoelectric focusing

Abstract

The specific conductivities of focused Ampholine, Servalyte and Pharmalyte carrier ampholytes have been determined and the results used to establish selection rules for carrier ampholytes in analytical isoelectric focusing.

The buffer capacity of focused Pharmalyte carrier ampholytes has been measured. These data and literature data for the Ampholine and Servalyte systems were used to establish selection rules for carrier ampholytes in preparative isoelectric focusing.

By combining the conductivity data with buffer capacity data for the three carrier ampholyte systems, differences between the molecular weights of these systems were revealed.