A method for the detection of proteolytic enzymes in electropherograms

Abstract

As has been described earlier, it is possible to localize amylase on agar electropherograms exactly and with great sensitivity with the help of a "sandwich method". This method is generally applicable to enzymes, the substrates of which can be differentiated from their degradation products by precipitation with some chemical agent. Therefore, the method can also be applied to the localization of protein-degrading enzymes, by precipitating the undegraded substrate protein with trichloroacetic acid.