Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae: a leap into disease pathophysiology
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Publication date
2017-07-01
Authors
Welle, Thijs
Hoekstra, Anna T.
Daemen, Ineke
Berkers, Celia R.
Costa, Matheus de O.
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Document Type
Article
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Abstract
Introduction: Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available. Objective: The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae. Methods: Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes. Results: Significant epithelial necrosis, increased levels of l-citrulline and IL-1α were observed on explants infected with B. hyodysenteriae. Conclusions: The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1α and NO.
Keywords
Brachyspira, Host-pathogen interactions, In vitro organ culture, Nitric oxide, Pathophysiology, Swine dysentery, Endocrinology, Diabetes and Metabolism, Biochemistry, Clinical Biochemistry
Citation
Welle, T, Hoekstra, A T, Daemen, I A J J M, Berkers, C R & de Oliveira Costa, M 2017, 'Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae : a leap into disease pathophysiology', Metabolomics, vol. 13, no. 7, 83. https://doi.org/10.1007/s11306-017-1219-6