High-Throughput Screening Assay Identifies Small Molecules Capable of Modulating the BMP-2 and TGF-β1 Signaling Pathway
Publication date
2017-01
Authors
Ghebes, Corina-Adriana
van Lente, Jéré
Post, Janine Nicole
Saris, Daniël B F
Fernandes, Hugo
Editors
Advisors
Supervisors
Document Type
Article
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cc_by_nc_nd
Abstract
Modulating the bone morphogenetic protein 2 (BMP-2) and transforming growth factor-β1 (TGF-β1) signaling pathways is essential during tendon/ligament (T/L) healing. Unfortunately, growth factor delivery in situ is far from trivial and, in many cases, the necessary growth factors are not approved for clinical use. Here we used a BMP-2 and a TGF-β1 reporter cell line to screen a library of 1280 Food and Drug Administration-approved small molecules and identify modulators of both signaling pathways. We identified four compounds capable of modulating BMP and TGF signaling on primary human tendon-derived cells (huTCs) and describe their effects on proliferation and differentiation of these cells.
Keywords
MP-2 signaling, TGF-β1 signaling, small-molecule discovery, tendon/ligament, BMP-2 signaling, Drug Discovery, Analytical Chemistry, Molecular Medicine, Biochemistry, Biotechnology, Pharmacology, Journal Article
Citation
Ghebes, C-A, van Lente, J, Post, J N, Saris, D B F & Fernandes, H 2017, 'High-Throughput Screening Assay Identifies Small Molecules Capable of Modulating the BMP-2 and TGF-β1 Signaling Pathway', Journal of biomolecular screening, vol. 22, no. 1, pp. 40-50. https://doi.org/10.1177/1087057116669346