Chemoenzymatic synthesis of the oligosaccharide moiety of the tumor-associated antigen disialosyl globopentaosylceramide
Publication date
2019-07-24
Authors
't Hart, Ingrid M E
Li, Tiehai
Wolfert, Margreet A
Wang, Shuo
Moremen, Kelley W
Boons, Geert-Jan
Editors
Advisors
Supervisors
Document Type
Article
Metadata
Show full item recordCollections
License
taverne
Abstract
Disialosyl globopentaosylceramide (DSGb5) is often expressed by renal cell carcinomas. To investigate properties of DSGb5, we have prepared its oligosaccharide moiety by chemically synthesizing Gb5 which was enzymatically sialylated using the mammalian sialyltransferases ST3Gal1 and ST6GalNAc5. Glycan microarray binding studies indicate that Siglec-7 does not recognize DSGb5, and preferentially binds Neu5Acα(2,8)Neu5Ac containing glycans.
Keywords
Taverne
Citation
't Hart, I M E, Li, T, Wolfert, M A, Wang, S, Moremen, K W & Boons, G-J 2019, 'Chemoenzymatic synthesis of the oligosaccharide moiety of the tumor-associated antigen disialosyl globopentaosylceramide', Organic & Biomolecular Chemistry, vol. 17, no. 31, 1368g, pp. 7304-7308. https://doi.org/10.1039/c9ob01368g