Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry
Publication date
2024
Authors
Deslignière, Evolène
Yin, Victor C
Ebberink, Eduard H T M
Rolland, Amber D
Barendregt, Arjan
Wörner, Tobias P
Nagornov, Konstantin O
Kozhinov, Anton N
Fort, Kyle L
Tsybin, Yury O
Editors
Advisors
Supervisors
Document Type
Article
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License
taverne
Abstract
Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to enable mass analysis of even highly heterogeneous, high-mass macromolecular assemblies. For contemporary Orbitrap instruments, the accessible ion detection (recording) times are maximally ~1-2 s. Here by modifying a data acquisition method on an Orbitrap ultrahigh mass range mass spectrometer, we trapped and monitored individual (single) ions for up to 25 s, resulting in a corresponding and huge improvement in signal-to-noise ratio (×5 compared with 1 s), mass resolution (×25) and accuracy in charge and mass determination of Orbitrap-based charge detection mass spectrometry.
Keywords
Taverne, Molecular Biology, Biochemistry, Biotechnology, Cell Biology
Citation
Deslignière, E, Yin, V C, Ebberink, E H T M, Rolland, A D, Barendregt, A, Wörner, T P, Nagornov, K O, Kozhinov, A N, Fort, K L, Tsybin, Y O, Makarov, A A & Heck, A J R 2024, 'Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry', Nature Methods, vol. 21, no. 4, pp. 619-622. https://doi.org/10.1038/s41592-024-02207-8