Studies on the mechanism of replication of adenovirus DNA. IV. Discontinuous DNA chain propagation

Abstract

The replication of adenovirus type 5 DNA occurs by discontinuous chain propagation via short pieces of DNA. These pieces accumulate if the infected cells are treated with hydroxyurea. They have a sedimentation coefficient of 11 S corresponding to a molecular weight of about 700,000, and they contain sequences derived from all parts of the adenovirus genome. Annealing and hybridization studies show that the short pieces represent sequences complementary to both strands of adenovirus type 5 DNA.