Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes
Publication date
2020-07-02
Authors
Buikema, Jan W
Lee, Soah
Goodyer, William R
Maas, Renee G C
Chirikian, Orlando
Li, Guang
Miao, Yi
Paige, Sharon L
Lee, Daniel
Wu, Haodi
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.
Keywords
GSK3, Wnt signaling, cardiomyocytes, expansion, induced pluripotent stem cells, maturation, proliferation, Taverne, Journal Article
Citation
Buikema, J W, Lee, S, Goodyer, W R, Maas, R G, Chirikian, O, Li, G, Miao, Y, Paige, S L, Lee, D, Wu, H, Paik, D T, Rhee, S, Tian, L, Galdos, F X, Puluca, N, Beyersdorf, B, Hu, J, Beck, A, Venkamatran, S, Swami, S, Wijnker, P, Schuldt, M, Dorsch, L M, van Mil, A, Red-Horse, K, Wu, J Y, Geisen, C, Hesse, M, Serpooshan, V, Jovinge, S, Fleischmann, B K, Doevendans, P A, van der Velden, J, Garcia, K C, Wu, J C, Sluijter, J P G & Wu, S M 2020, 'Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes', Cell stem cell, vol. 27, no. 1, pp. 50-63.e5. https://doi.org/10.1016/j.stem.2020.06.001