Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae
Publication date
2025-07-02
Authors
Rajakani, Sahaya Glingston
Xavier, Basil Britto
Nguyen, Ngoc Minh
Lin, Qiang
Braspenning, Anouk
Plantinga, Nienke L
Wittekamp, Bastiaan H J
Zarkotou, Olympia
Van Houdt, Rob
Glupczynski, Youri
Editors
Advisors
Supervisors
Document Type
Article
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Abstract
Colistin is one of the last-line antibiotics against multi-drug-resistant (MDR) gram-negative pathogens, such as Klebsiella pneumoniae. Using long-read sequencing, we observed remarkable genome-wide transposition events in MDR K. pneumoniae exposed to colistin in patients receiving treatment for respiratory infections or as part of selective decolonization strategies and further confirmed these on in vitro selection experiments. These data add yet another dimension to the role of antibiotics in mediating specific processes in bacteria beyond antibiotic resistance.
Keywords
antibiotic resistance, colistin, insertion sequence elements, muti-drug resistant Klebsiella penumoniae, Journal Article
Citation
Rajakani, S G, Xavier, B B, Nguyen, N M, Lin, Q, Braspenning, A, Plantinga, N L, Wittekamp, B H J, Zarkotou, O, Van Houdt, R, Glupczynski, Y, Pournaras, S, Bonten, M J M & Malhotra-Kumar, S 2025, 'Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae', Antimicrobial Agents and Chemotherapy, vol. 69, no. 7, e0157424. https://doi.org/10.1128/aac.01574-24